Journal: Drug Design, Development and Therapy

Article Title: Liraglutide attenuates cardiac remodeling and improves heart function after abdominal aortic constriction through blocking angiotensin II type 1 receptor in rats

doi: 10.2147/DDDT.S213910

Figure Lengend Snippet: Effects of liraglutide and telmisartan on the expression of AT1R, AT2R, AT1R/AT2R ratio, and ACE2 after AAC. ( A and B ) Protein levels of AT1R and AT2R were detected by Western blot assay. ( C ) AT1R/AT2R ratio was calculated from the intensity of each individual bands. ( D ) ACE2 expression in the intermyocardium and intracardiac vessels was determined using immunohistochemical staining and calculated as mean optical density (MOD) in the tissue section (magnification: 200; scale bars: 100 μm). Values are mean±SEM (n=6/group). * p <0.05 AAC vs Sham; # p <0.05 Lira or Telmi vs AAC. Abbreviation: AAC, abdominal aortic constriction.

Article Snippet: The protein contents of the AT1R and AT2R, TGF-β1, Smads, and collagens were determined by Western blot assay., In brief, the heart tissue samples from different groups were homogenized in a lysis buffer and the protein concentration was measured by the BCA Protein Assay reagent kit (Boster Biotech, Wuhan, People’s Republic of China).

Techniques: Expressing, Western Blot, Immunohistochemical staining, Staining

Journal: International Journal of Molecular Sciences

Article Title: Megalin, Proton Pump Inhibitors and the Renin–Angiotensin System in Healthy and Pre-Eclamptic Placentas

doi: 10.3390/ijms22147407

Figure Lengend Snippet: Gene expression levels of RAS components ( A – G ) and megalin ( H ) in healthy and early-onset pre-eclamptic (EoPE) placental tissue. ( I , J ): AGT expression was confirmed with different pairs of primers. Data are mean ± SEM of n = 12. * p < 0.05. ( P)RR , (pro)renin receptor; AGT , angiotensinogen; ACE , angiotensin-converting enzyme; AT1R , angiotensin II type 1 receptor; AT2R , angiotensin II type 2 receptor; A.U., arbitrary unit.

Article Snippet: The blots were then probed with antibodies against angiotensinogen (1:100, Abbiotec, Shenzhen, China), GAPDH (1:5000, GeneTex, Hsinchu, China), β-actin (1:5000, Merck Millipore, Darmstadt, Germany), or megalin (1:500, Biotech, Wuhan, China), and detected by using Clarity Western ECL Substrate (Bio-Rad).

Techniques: Gene Expression, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Megalin, Proton Pump Inhibitors and the Renin–Angiotensin System in Healthy and Pre-Eclamptic Placentas

doi: 10.3390/ijms22147407

Figure Lengend Snippet: Total renin ( A ), renin ( B ) and prorenin ( C ) levels ( n = 16) measured by immunoradiometric assay and angiotensinogen (AGT; D , E ) and megalin ( F , G ) protein abundance ( n = 12) measured by Western blot in healthy and early-onset pre-eclamptic (EoPE) placental tissue. Panels ( D , F ) are representative blots of AGT and megalin protein. Data were normalized versus GAPDH ( E ) or β-actin ( G ). Data are mean ± SEM. * p < 0.05, ** p < 0.005, *** p < 0.0005 vs. Healthy. AGT, angiotensinogen.

Article Snippet: The blots were then probed with antibodies against angiotensinogen (1:100, Abbiotec, Shenzhen, China), GAPDH (1:5000, GeneTex, Hsinchu, China), β-actin (1:5000, Merck Millipore, Darmstadt, Germany), or megalin (1:500, Biotech, Wuhan, China), and detected by using Clarity Western ECL Substrate (Bio-Rad).

Techniques: Immunoradiometric Assay, Quantitative Proteomics, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Megalin, Proton Pump Inhibitors and the Renin–Angiotensin System in Healthy and Pre-Eclamptic Placentas

doi: 10.3390/ijms22147407

Figure Lengend Snippet: Prorenin ( A ), renin ( B ) and angiotensinogen ( C ) release from isolated perfused healthy ( n = 5) and early-onset pre-eclamptic (EoPE, n = 6) cotyledons. AGT: angiotensinogen. Perfusate collection from two of the pre-eclamptic placentas could not be continued over the full 180 min due to fetal-to-maternal leakage. In such cases, only the samples prior to the occurrence of leakage were used. Data are mean ± SEM. * p < 0.05.

Article Snippet: The blots were then probed with antibodies against angiotensinogen (1:100, Abbiotec, Shenzhen, China), GAPDH (1:5000, GeneTex, Hsinchu, China), β-actin (1:5000, Merck Millipore, Darmstadt, Germany), or megalin (1:500, Biotech, Wuhan, China), and detected by using Clarity Western ECL Substrate (Bio-Rad).

Techniques: Isolation

Journal: International Journal of Molecular Sciences

Article Title: Megalin, Proton Pump Inhibitors and the Renin–Angiotensin System in Healthy and Pre-Eclamptic Placentas

doi: 10.3390/ijms22147407

Figure Lengend Snippet: Schematic overview. AGT, angiotensinogen; , inhibition; PPI, proton pump inhibitor; (P)RR, (pro)renin receptor; Ang I, angiotensin I; ACE, angiotensin-converting enzyme; Ang II, angiotensin II; AT1R, angiotensin II type 1 receptor; PE, pre-eclampsia; sFlt-1, soluble Fms-like tyrosine kinase-1; +, normal sensitivity to Ang II; +++, increased sensitivity to Ang II.

Article Snippet: The blots were then probed with antibodies against angiotensinogen (1:100, Abbiotec, Shenzhen, China), GAPDH (1:5000, GeneTex, Hsinchu, China), β-actin (1:5000, Merck Millipore, Darmstadt, Germany), or megalin (1:500, Biotech, Wuhan, China), and detected by using Clarity Western ECL Substrate (Bio-Rad).

Techniques: Inhibition

Journal:

Article Title: Estrogen prevents intestinal inflammation after trauma-hemorrhage via downregulation of angiotensin II and angiotensin II subtype I receptor

doi: 10.1152/ajpgi.90443.2008

Figure Lengend Snippet: Angiotensin II (Ang II) level in plasma and small intestine in sham and trauma-hemorrhage animals treated with vehicle (Veh), 17β-estradiol (E2), and ICI 182,780 (ICI) + E2 (A and B), and Veh or Ang II subtype-I receptor (AT1R) antagonist losartan (Los) (C and D). Data are shown as means ± SE of 5 animals in each group. *P < 0.05 compared with sham or trauma-hemorrhage + E2.

Article Snippet: The Western blot transfers were immunoblotted with anti-rat AT1R, followed by the addition of horseradish peroxidase-conjugated secondary antibody for detection of bound antibody by enhanced chemiluminescence (Amersham Pharmacia Biotech, Piscataway, NJ).

Techniques:

Journal:

Article Title: Estrogen prevents intestinal inflammation after trauma-hemorrhage via downregulation of angiotensin II and angiotensin II subtype I receptor

doi: 10.1152/ajpgi.90443.2008

Figure Lengend Snippet: AT1R protein expression in small intestine from sham and trauma-hemorrhage animals treated with Veh, Los, E2, or ICI + E2. For equal protein loading, membranes were probed for GAPDH using mouse anti-GAPDH monoclonal antibody. Representative blot is shown in A. Blots were analyzed densitometrically; the densitometric values were pooled from 4 animals in each group and are shown in B. Data are expressed as means ± SE and compared by one-way ANOVA and Tukey's test: *P < 0.05 vs. sham or trauma-hemorrhage + E2.

Article Snippet: The Western blot transfers were immunoblotted with anti-rat AT1R, followed by the addition of horseradish peroxidase-conjugated secondary antibody for detection of bound antibody by enhanced chemiluminescence (Amersham Pharmacia Biotech, Piscataway, NJ).

Techniques: Expressing

Journal: Arthritis

Article Title: Expression of Angiotensin II Receptor-1 in Human Articular Chondrocytes

doi: 10.1155/2012/648537

Figure Lengend Snippet: Expression of angiotensin receptors in human chondrocytes. Representative results of RT-PCR using 5 OA, 5 RA, and 4 fracture (control) samples are shown. AT1R: 255 bp, AT2R: 191 bp, GAPDH: 598 bp.

Article Snippet: Rabbit polyclonal anti-human AT1R antibodies (Abs) were purchased from Assay Designs (Ann Arbor, MI, USA; for immunohistochemistry) and from Alomone Labs, Ltd. (Jerusalem, Israel; for western blotting and flow cytometry).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control

Journal: Arthritis

Article Title: Expression of Angiotensin II Receptor-1 in Human Articular Chondrocytes

doi: 10.1155/2012/648537

Figure Lengend Snippet: Immunohistochemical analysis of AT1R expression in human cartilage. Representative results are shown. (a) Original magnification: ×4. (b) An OA sample (×40). (c) An RA sample (×40).

Article Snippet: Rabbit polyclonal anti-human AT1R antibodies (Abs) were purchased from Assay Designs (Ann Arbor, MI, USA; for immunohistochemistry) and from Alomone Labs, Ltd. (Jerusalem, Israel; for western blotting and flow cytometry).

Techniques: Immunohistochemical staining, Expressing

Journal: Arthritis

Article Title: Expression of Angiotensin II Receptor-1 in Human Articular Chondrocytes

doi: 10.1155/2012/648537

Figure Lengend Snippet: IL-1-induced upregulation of AT1R/AT2R mRNA expression in chondrocytes. Results of the quantitative PCR analyses are shown. (a) Normal ( n = 3) (b) OA ( n = 4) (c) RA ( n = 4).

Article Snippet: Rabbit polyclonal anti-human AT1R antibodies (Abs) were purchased from Assay Designs (Ann Arbor, MI, USA; for immunohistochemistry) and from Alomone Labs, Ltd. (Jerusalem, Israel; for western blotting and flow cytometry).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Arthritis

Article Title: Expression of Angiotensin II Receptor-1 in Human Articular Chondrocytes

doi: 10.1155/2012/648537

Figure Lengend Snippet: AT1R protein expression in chondrocytes. (a) Western Blotting . Chondrocytes with or without IL-1 stimulation were analyzed: AT1R: approximately 60 kD (see text), GAPDH: 36 kD. (b) Flow Cytometry . A representative result is shown, shaded area: control staining, solid line: nonstimulated chondrocytes, dotted line: IL-1-stimulated chondrocytes. Representative results of 2 OA samples are shown.

Article Snippet: Rabbit polyclonal anti-human AT1R antibodies (Abs) were purchased from Assay Designs (Ann Arbor, MI, USA; for immunohistochemistry) and from Alomone Labs, Ltd. (Jerusalem, Israel; for western blotting and flow cytometry).

Techniques: Expressing, Western Blot, Flow Cytometry, Control, Staining